Uridine 2 -deoxy-, 5 - , enzymic synthesis

Hexokinase catalysed syntheses of 6-amino-6-deoxy-D-glucose and -D-gluconate 6-phosphates, 6-amino-6-deoxy-D-fructose 6-phosphate and 1,6-bisphosphate, and 5-amino-5-deoxy-D-ribulose 5-phosphate have been reported. From a study of the properties and reactivity in enzymic reactions of these compounds, it was concluded that they were excellent isosteric analogues of the normal metabolic intermediates except for reactions catalysed by kinases. A preparative enzymic synthesis of uridine 5 -diphospho-2-acetamido-2-deoxy-D-glucose used chemically synthesized 2-acetamido-2-deoxy-D-glucose 1-phosphate and uridine triphosphate as reactants, and a calf liver enzyme extract containing uridine-5 -phospho-A-acetylglucosamine pyrophosphorylase. ... 

One-pot synthesis of nucleotide sugars was also demonstrated for multi-enzyme systems as shown for UDP-Gal [107, 108] and dTDP-2-deoxy-Glc [109]. Even some modified sugar nucleotides were synthesized in an one-pot fashion, namely UDP-carba-sugars based on Gal, GlcNAc, Glc, and Man [110]. The cascade involved uridine monophosphate (UMP)-kinase and acetate kinase to generate uridine triphosphate (UTP) as well as Glc-l-P-uridyltransferase, Gal-l-P-uridyltransferase,... 

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