Ultraviolet visible microspectrophotometry

Other useful microscopic analytical techniques include hot stage, fluorescence, and cathodolumines-cence microscopies micro-infrared spectroscopy micro-Raman spectroscopy ultraviolet-visible microspectrophotometry and X-ray diffraction however, the discussion of these techniques is beyond the scope of this article. Briefly stated, each of these techniques can be used to ascertain additional information about characteristic properties of a material. The microscopist must be aware of all of these techniques, and others, so as to be able to extract the necessary information from a sample when the need arises. 

Ultraviolet/Visible Microspectrophotometry. Infrared spectroscopy and ultraviolet/visible microspectrophotometry are both based on the principle of the interaction of radiation with a sample. However, ultraviolet/visible microspectrophotometry is typically used to compare the dye or pigment composition of samples. The technique is used to determine the color of a sample and identify subtle differences in color that cannot be seen with the naked eye. 

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