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Ultrathin sectioning immunolabeling

After glutaraldehyde fixation (Section 3.1 1 2., step 8), fix the cells further with 1% osmium tetroxide, saturated uranyl acetate, dehydrate in an ascending series of ethanol (70, 90, 100%), and embed in epoxy resin. Ultrathin sections of the block, stained with 1% methanolic uranyl acetate and lead citrate, will reveal immunolabeling on the outer surface of the cells... [Pg.305]

Sectioning Ultrathin sections of LR gold-embedded specimens are cut as for standard (epoxy) electron microscopy resins. Cut sections fresh for any immunolabel-ing experiment. Note Collect sections on nickel grids (300 mesh). Grids may be nsed with or without Formvar coating. [Pg.66]

Slot, J.W., and Geuze, H.J. (1984) Cold markers for single and double immunolabeling of ultrathin cryo-sections. In Immunolabeling for Electron Microscopy (J.M. Polak, and I.M. Varndess, eds.), p. 139. Elsevier, New York. [Pg.1116]

See other pages where Ultrathin sectioning immunolabeling is mentioned: [Pg.99]    [Pg.99]    [Pg.306]    [Pg.182]    [Pg.20]    [Pg.257]    [Pg.84]    [Pg.85]    [Pg.91]    [Pg.93]    [Pg.105]    [Pg.378]    [Pg.382]    [Pg.384]    [Pg.386]    [Pg.304]    [Pg.180]    [Pg.289]    [Pg.377]    [Pg.385]   
See also in sourсe #XX -- [ Pg.84 ]



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