Ultrastyragel columns separation

Figure 1. Separation of BHA and BHT on a lOOA Ultrastyragel column using chloroform and tetrahydrofuran. Conditions ...

FIGURE 11-2. Separation of mixture of phthalates by GPC on a 100-A Ultrastyragel column. Analytical loadings are shown in (a) and preparative loadings are shown in (b). Mobile phase THF. Flow rate 1 mL/min. Detector UV at 254 nm, 0.5 AUFS in (a) and 2.0 AUFS in (fe) RI at X8 in (a) and X64 in (b). Sample dioctyl, dibutyl, and dimethyl phthalates with 50 /xL injection in (a) and 100-/xL injection in (b). (Note Actual separation will depend upon the quality of the mobile phase and column packing). 

FIGURE 5-38. The effect of mobile phase upon detector response. The separation is a mixture of n-hydrocarbons on two columns 100 A ultrastyragel, 7.8 mm x 30 cm) using an RI detector. Individual hydrocarbon peaks Qo (limited solubility), C32, C24, C 8, C12, C7, and C5. Mobile phase (a) tetrahydrofuran (b) toluene and (c) methylene chloride. (The peak for C24 is missing since the RI for C24 is similar to that of methylene chloride.)... 

FIGURE 5-42. Separation of nonanedioic acid from octanoic acid using SMGPC. (a) A 100-p.L injection containing 10 mg of each acid. (b) A 1-mL injection containing 10 mg of each acid, (c) A 1-mL injection containing 100 mg of each acid (note overload of RI detector), (d) Material from the second peak in (c) collected and reinjected. Column 100 A Ultrastyragel, 7.8 mm ID x 30 cm. (Reprinted from reference 9 with permission.)... 

B. Typical chromatograms for the separation of dimethyl, dibutyl, and dioctyl phthalates on one 100-A /aStyragel column are shown in Figure 11-1 and for the 100-A Ultrastyragel in Figure 11-2. 

Warren [4] showed a determination of warfarin in rodent bait. The bait is of higher molecular mass and is clearly separated from warfarin in a 12-minute analysis on a single Ultrastyragel 100 A column with tetrahydrofuran as solvent. 

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