Ultracentrifuge optical system, figure

Ultracentrifugation 108,109 Ultracentrifuge 100 analytical 108 optical system, figure 109 Ultrafiltration 100 Ultrasensitive responses 567 Ultrasonic sound 468 UMP (Uridine5-phosphate) 200, 200s,203 Unimolecular processes 457 Uniporters 414 Units, International System 2 Unsatisfied ends of hydrogen bonded chains 64... 

Figure 3-7 The scanning absorption optical system of the Beckman Optima XL-A ultracentrifuge. Courtesy of Beckman Coulter.

Figure 2 Sedimentation velocity analysis of the interaction between bisANS and bacteriophage P22 coat protein. Coat protein in the presence of 30 pM bisANS Co = 0.3 mg/ml The right hand ordinate has units of mg-ml -svedberg. Sedimentation was carried out at 56,000 rpm at 20 °C t = 7304 sec in a Beckman XL-A ultracentrifuge equipped with a photoelectric scanner and video-based on-line Rayleigh optical system similar to the one installed on the Model-E but using a high resolution Kodak MegaPlus 1.4 digital camera (Stafford, to be described elsewhere).

Other optical techniques have also been used to monitor analytical ultracentrifugation experiments. These included refractive index (Schlieren optics) as well as interference patterns produced using Rayleigh- or Lebedev-type optics.11 In both cases, data are acquired using photographic or video recording equipment. Examples of data obtained with these different optical systems are shown in Figure 13.11. 

Figure 5.5.1 Schematic of a modern analytical ultracentrifuge with scanning absorption optical system. [After Cantor, C.R. Schimmel, P.R. 1980. Biophysical Chemistry. Part II Techniques for the Study of Biological Structure and Function. San Francisco W.H. Freeman. Copyright 1980 W.H. Freeman and Company. With permission.]...

Figure 13.8. Analytical ultracentrifuge equipped with a UV-vis optical detection system. The sliding slit at the photomultiplier allows positional recording of the absorbance along the cell.

Figure 13.11. Results for a moving-boundary ultracentrifuge experiment using different optical detection systems and a double-sector cell. Part (a) is a graphical representation, (b) is the result of an uv photograph, (c) is a plot of absorbance versus distance (from b), id) is a photograph obtained with Schlieren optics, (e) is an interference diagram obtained using Rayleigh optics, and (f) is another interference diagram, obtained with Lebedev optics.

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