Ultra Performance Convergence Chromatography A New Synthesis

Waters BEH UPLC C18 RP-column and Acquity UPC BEH column with UV detection. (Used with permission from Waters Corp., Milford, MA, www.waters.com/upc2.) 

Toward the end of Section 11.5, we saw that one of the many useful features of fused silica capillary tubing was the ability of its surface silanol groups to lose ions and form a chemically bound layer of 

Pep is positive for cations and negative for anions, so p pp will be greater or less than Pe. The apparent mobility of an ion and the electrophoretic mobility can be calculated according to 

Although we have emphasized that the fundamental principle of electrophoretic separation is different from that of chromatography, there are many similarities in the appearance of the separation. There is sample injection, flow of a carrier fluid along a column, similar detectors employed near the end of the column, and separated compounds being displayed as peaks emerging at characteristic times. Similar formulas can be used to describe the resolution of separations and to 

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