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Tyrosyl-lysine to BSA

Conjugation of Tyrosyl-Lysine to BSA Using Various Amounts of EDC... [Pg.759]

Figure 19.12 EDC conjugation reactions can be extraordinarily consistent using the same peptide crosslinked to two carrier proteins. This figure shows the gel filtration pattern on Sephadex G-25 after completion of the crosslinking reaction. Conjugation of tyrosyl-lysine to BSA and KLH are shown. The first peaks represent eluting carrier, while the second peaks are the excess peptide. Note the consistency of conjugation using the same levels of EDC addition. Figure 19.12 EDC conjugation reactions can be extraordinarily consistent using the same peptide crosslinked to two carrier proteins. This figure shows the gel filtration pattern on Sephadex G-25 after completion of the crosslinking reaction. Conjugation of tyrosyl-lysine to BSA and KLH are shown. The first peaks represent eluting carrier, while the second peaks are the excess peptide. Note the consistency of conjugation using the same levels of EDC addition.
Figure 19.8 To study the conjugation of peptides to carriers using different levels of EDC, tyrosyl-lysine was conjugated to BSA and separated after the reaction by chromatography on a Sephadex G-25 column. As the EDC level was increased in the reaction, more peptide reacted and the peptide peak (the second peak) was depleted. The absorbance of the carrier peak (the first one) increases as more peptide is conjugated. Figure 19.8 To study the conjugation of peptides to carriers using different levels of EDC, tyrosyl-lysine was conjugated to BSA and separated after the reaction by chromatography on a Sephadex G-25 column. As the EDC level was increased in the reaction, more peptide reacted and the peptide peak (the second peak) was depleted. The absorbance of the carrier peak (the first one) increases as more peptide is conjugated.
Comparison of Tyrosyl-Lysine Conjugation to BSA and KLH Using Various Concentrations of EDC... [Pg.761]

See other pages where Tyrosyl-lysine to BSA is mentioned: [Pg.757]    [Pg.452]    [Pg.432]    [Pg.757]    [Pg.452]    [Pg.432]    [Pg.453]    [Pg.433]   
See also in sourсe #XX -- [ Pg.757 ]



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