Tyrosyl-lysine, conjugation

Comparison of Tyrosyl-Lysine Conjugation to BSA and KLH Using Various Concentrations of EDC... 

Figure 19.11 shows the conjugation of tyrosyl-lysine to KLH using various concentrations of EDC. The elution profile shows the gel filtration pattern resulting after the reaction. Progressive decrease in the peptide peak (peak 2) with increasing amounts of EDC correlates to... 

Conjugation of Tyrosyl-Lysine to BSA Using Various Amounts of EDC... 

Figure 19.8 To study the conjugation of peptides to carriers using different levels of EDC, tyrosyl-lysine was conjugated to BSA and separated after the reaction by chromatography on a Sephadex G-25 column. As the EDC level was increased in the reaction, more peptide reacted and the peptide peak (the second peak) was depleted. The absorbance of the carrier peak (the first one) increases as more peptide is conjugated.

Figure 19.11 The EDC conjugation of tyrosyl-lysine to KLH is illustrated by the gel filtration pattern on Sephadex G-25 after the reaction. The first peak is the carrier protein and the second peak is the peptide. A blank containing no EDC is also shown to provide baseline peak heights that would be obtained if no crosslinking occurred. When more EDC was added, more peptide was conjugated, as evidenced by peptide peak depletion.

Figure 19.12 EDC conjugation reactions can be extraordinarily consistent using the same peptide crosslinked to two carrier proteins. This figure shows the gel filtration pattern on Sephadex G-25 after completion of the crosslinking reaction. Conjugation of tyrosyl-lysine to BSA and KLH are shown. The first peaks represent eluting carrier, while the second peaks are the excess peptide. Note the consistency of conjugation using the same levels of EDC addition.

Capromab pendetide is the murine MAb, 7E11-C5.3, conjugated to the linker-chelator, glycyl-tyrosyl-(A/-diethylenetriaminepentaacetic acid)-lysine hydrochloride (GYK-DTPA-HCI). Given with Indium In 111. 

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