Two-Component Anionic Lipid Models with Sink Condition in the Acceptor Compartment

3 Two-Component Anionic Lipid Models with Sink Condition in the Acceptor Compartment 

Since there would be increased overall lipid concentration in the dodecane solution, we decided to create a sink condition in the acceptor wells, to lower the membrane retention. We discovered that the pH 7.4 buffer saturated with sodium laurel sulfate serves as an excellent artificial sink-forming medium. Since the new PAMPA membranes would possess substantial negative charge, the negatively charged micellar system was not expected to act as an aggressive detergent to the two-component artificial membrane infused in the microfilter. 

Six two-component models were tested under sink conditions (models 5.1-10.1 in Table 7.3), employing three negatively charged lipids (dodecylcarboxylic acid, phosphatidic acid, and phosphatidylglycerol). These models were also tested in the absence of the sink condition (models 5.0-10.0 in Table 7.3). 

TABLE 7.6 Two-Component Anionic Lipid PAMPA Models (with Sink), pH lAa 

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