Tryptic digest peptides, elution profile

Fig. 5. Elution profiles obtained for the tryptic digest peptides of hemoglobin A using three distinct gradient shapes (number 3,6, and 8 on the Waters M660 solvent programmer). In each case, two /u-Bondapak C, columns were connected in series, a flow of 1.7 ml/min was used, and a 30-min linear gradient was generated from solvent A (0.phosphoric acid, pH 2.2) and solvent B (SOOt acetonitrile-0.1% phosphoric acid, pH 2.2). Reprinted with permission from Bishop et al. (5. ). Copyright by Marcel Dekker, Inc., New York.

Fig. 2. Reverse phase HPLC fractionation of a tryptic digest of Ado-P..-[ H]P1 modified PRK. Sample (1.9 nmol)was loaded onto a LiChrospher RP-18 column and eluted with a 0-70% gradient of CH CN in 0.1% trifluoroacetic acid. The top panel shows 215 nm absorbance, with the inset depicting a 20-fold scale expansion to indicate (arrow) the UV peak corresponding to the radiolabeled peptide. The bottom panel shows the H profile measured using 10% of the column effluent.

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