Trypsin streptokinase

Streptokinase Trypsin Uiicase Urokinase Plasminogen- plasmin Protein hydrolysis Urate+02->allantoin Plasminogen->plasmm Blood clots Inflammation Gout Blood clots... 

At present, the binary water-soluble preparation of heparin and proteolytic enzymes is being applied for the treatment of thromboses. For instance, injection into the bloodstream of heparin-plasmin complex or a heparin-plasmin-streptokinase preparation leads to the total dissolution of the thrombus, while if introduced separately, heparin and streptokinase do not display the lytic action at all, and plasmin, alone or together with streptokinase, dissolves the thrombus only partially 132>. The treatment of acute thrombophlebitis with trypsin resulted in a full dissolution of the thrombus and in an increase of antithrombin III in the blood 133). Administration of trypsin together with heparin has an effect similar in efficiency to the action of the heparin-plasmin complex 134>. The use of a mix of heparin and urokinase for improving tbrom-boresistance of polymeric materials was also described 13S). These substances were immobilized by preliminary coating of the surface of a polymer with a graphite layer and subsequent adsorption of heparin and the enzyme. 

Epsilon-Amino-n-Caproic Acid (EACA), C.P., homogeneous (Mann Research Labs.) in concentration of O.IM 0.4 ml per experiment was added. Plasminogen, human, purified (Mann Research Labs.) in concentration of 1 mg/ml VS (15 RPMI units/mg) 0.4 ml was added per experiment. Approximately 44-45 unit amounts (dissolved in VS) of Streptokinase (SK), 100,000 Christensen units/vial. 0.1 mg = 400 units (Lederle Laboratories) was used per experiment. Trypsin Inhibitor, Soybean (SBTI), chromatographic (component VI, salt free, lyoph.), 10,000 BAEE units of inhibition per mg (Mann Research Labs.), was... 

After the first substitution, the remaining chloride of 31 is reactive enough for a second substitution, and this can lead to undesirable crosslinking of the proteins. Several examples of the use of 31 have been published, mainly in early studies, including the PEGylation of ovoalbumin [137,138], bovine serum albumin [20], superoxide dismutase [139], elastase [140], acyl-plasmin-streptokinase complex [141], trypsin [142], silk fibroin [143] and immunoglobulin [144], catalase [145,146], amongst others. 

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