Troubleshooting problems encountered during immunoblotting

1 Problems associated with eiectrotransfer from i to membrane 

3 Signals due to cross ieactivity of the primary or secondary antibodies with other proteins 

Appropriate positive and negative controls should be included to confirm that the signals detected are due to specific recognition of the target antigen  

Optimization of the conditions during electrotransfer and immunoblotting is discussed in ref. 6. 

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