Triphasic column

Kobayashi, J. and Mori, Y. and Kobayashi, S. (2005). Triphase Hydrogenation Reaetions Utilizing Palladium-Immobilized Capillaiy Column Reaetors and a Demonstration of Suitability for Large Scale Synthesis. Adv. Synth. Catal, 347, 1889-1892. 

The MudPIT procedure is a powerful approach to comprehensive proteome mining. In a comparison of the biphasic MudPIT approach (Figure 18. la) with either ID-RPLC-MS or triphasic MudPIT (Figure 18. Ib), 26, 55, and 62 proteins were identified from a complex protein mixture associated with bovine brain microtubules using the ID, 2D, and 3D approach, respectively [46]. The 3D approach enabled the identification of some hydrophilic peptides that were missed by the 2D approach. Even a tetraphasic MudPIT with longer columns was described [47]. 

From the analytical point-of-view, developments in this field comprise further improvements in the chromatographic separation efficiency, as for instance demonstrated by the use of a triphasic MudPIT approach [46], and of in-needle columns packed with 1-pm particles, and operated at flow-rates of <50 nl/min [122]. 

Kobayashi, J., Y. Mori, and S. Kobayashi, Triphase hydrogenation reactions utilizing paUadiumimmo-bihzed capillary column reactors and a demonstration of snitabUity for large scale synthesis. Advanced Synthesis and Catalysis, 2005, 347 1889-1892. 

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