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Triose phosphate isomerase gene

Fig. 13.1 S. cerevisiae expression vector used for production of recombinant insulin. The S. cerevi-siae/E. coli shuttle vector is composed of the following genetic units 1) The transcription promoter and terminator of the S. cerevisiae TPIl (triose phosphate isomerase) gene flanking the DNA encoding the leader-insulin precursor. 2) The TPIl gene from Schizosaccharomyces pombe (TPIlp)... Fig. 13.1 S. cerevisiae expression vector used for production of recombinant insulin. The S. cerevi-siae/E. coli shuttle vector is composed of the following genetic units 1) The transcription promoter and terminator of the S. cerevisiae TPIl (triose phosphate isomerase) gene flanking the DNA encoding the leader-insulin precursor. 2) The TPIl gene from Schizosaccharomyces pombe (TPIlp)...
Deletion mutants, in which the bla signal sequence is almost completely removed, e.g., Met-Ser-(263 amino acid /8-lactamase) or Met-Arg-Ser-(263 amino acid /8-lactamase), produce but do not secrete the /8-lactamase (62). Note The extra 2 or 3 amino acids at the N terminus do not affect the catalytic activity of the enzyme.) The fusion protein expressed from the bla signal codons fused to an intracellular protein (e.g., triose-phosphate isomerase) gene is neither secreted into the periplasm of E. colt nor proteolytically processed. [Pg.608]


See other pages where Triose phosphate isomerase gene is mentioned: [Pg.141]    [Pg.141]    [Pg.1109]    [Pg.68]    [Pg.400]    [Pg.105]    [Pg.144]    [Pg.184]    [Pg.114]    [Pg.199]    [Pg.1109]    [Pg.235]    [Pg.351]    [Pg.807]    [Pg.500]    [Pg.84]    [Pg.995]    [Pg.84]    [Pg.1136]    [Pg.193]   
See also in sourсe #XX -- [ Pg.141 ]




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