2,5,8-trimethyl psoralen

Synonyms Psoralen and UVA Photochemotherapy Light therapy 8-Methoxypsoralen (8-MOP) 5-Methoxypsoralen (5-MOP) 4,5, 8-Trimethyl-psoralen (TMP)... 

Trimethyl psoralen Small deletions, average size of 3 kilobases Loss of function Small deletions affecting mostly single genes fewer rearrangements Null mutants only... 

Section 7.7.1) and HMT (4 -hydroxymethyl-4,5, 8-trimethyl-psoralen)furan thymidine adducts are formed by photofixation simultaneously with hybridization ( photochemical pumping ). Excess of probes is removed after hybridization in solution by Centricon cen-... 

Amino acid-modified furocoumarins were prepared by condensation of the T-hydroxysuccinimide ester of 3-(2,3,5-trimethyl-7-oxofuro[3,2-g]chromen-6-yl)propanoic acid 51 with amino acids <2003JPH177>. Ethyl 3-(7-hydroxy-4-methyl-2-oxo-2/7-3-chromenyl)propanoate 49, prepared in 56% yield by Pechmann condensation of resorcinol and diethyl 2-acetylglutarate in the presence of HCl, was condensed with 3-chlorobutan-2-one in the presence of base to give propanoate 50 (Scheme 4). The MacLeod method was then used to fuse a furan ring to the benzopyran-2-one system. Heating 50 with NaOH solution (1 M) readily cyclized it into the corresponding psoralen furocoumarin 51 with simultaneous hydrolysis of the ester. 

Adam, W., Beinhauer, A., Fisher, R., and Hauer, H., A psoralen-substituted dioxetane as DNA intercalator for photogenotoxic studies. Angew. Chem., Int. Ed. Engl. 26, 796-797 (1987). Adam, W., Bhushan, V., Fuchs, R., and Kirchgassner, U., Functionalized 1,2-dioxetanes as potential phototherapeutic agents. The synthesis of carboxylate, carbonate, carbamate, and ether derivatives of 3- (hydroxymethyl)-3,4,4-Trimethyl-l,2-dioxetane. J. Org. Chem. 52, 3059-3062 (1987). 

The interaction of MNPs with DNA is a well-known phenomenon [86-89], and single- or double-strand DNA is decorated by MNPs, which can easily be imaged by transmission electron microscopy (TEM) or AFM [90-92]. Various surface-functionalized AuNPs have been prepared to attach AuNPs securely to DNA molecules. In numerous studies, such AuNPs were prepared by surface modifications with cationic thiols [54, 55, 92] or intercalators [93, 94]. The ID chains of AuNPs coated with cationic trimethyl(mercaptoundecyl) ammonium monolayers were electrostatically assembled along DNA molecules in solution by the relative molar quantities of AuNPs and DNA base pairs. Since psoralen acts as a specific intercalator for A-T base pairs, the functionalized AuNPs were assembled onto the pA-pT ds-DNA [93]. Furthermore, UV irradiations induced the reaction between the psoralen units and the thymine of DNA, and then covalently fixed AuNPs to the DNA. 

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