Triiodo-thyronine

Purification The crude precipitate is dissolved in boiling 2N HCl (300 ml) and filtered from the relatively insoluble thyroxine hydrochloride. The hot filtrate is brought to pH 4 with 5N NaOH and triiodo thyronine again separates after chilling at 0° to 4°C it is collected, washed with water and dried. The yield of triiodo thyronine is 70 to 75% of the theoretical. This triiodo thyronine still contains some thyroxine (about 10%). 

The final purification consists of chromatographic separation of thyroxine and triiodo thyronine on a kieselguhr column using 20% chloroform in n-butanol equilibrated with... 

N NaOH as the developing solvent. 80 to 100 mg triiodo thyronine is purified during... 

The final purification consists of chromatographic separation of thyroxine and triiodo thyronine on a kieselguhr column using 20% chloroform in n-butanol equilibrated with 0.5N NaOH as the developing solvent. 80 to 100 mg triiodo thyronine is purified during each run on a 50 g kieselguhr column. Pure L-triiodo thyronine has MP 236° to 237°C (dec.) and [a]D29-5° = +21.5 in a 4.75% solution in a mixture of 1 part of N HCI and 2 parts of ethanol. Liothyronine is commonly used as the sodium salt. 

Figure 3 Reaction cycle catalyzed by the type I tetraiodothyronine deiodinase. T4 = 3,3, 5,5 -tetraiodothyronine T3 = 3,3, 5-triiodo-thyronine Au+ = gold thioglucose PU-SH = propylthiouracil...

L- 3, 5, 3 - triiodo - Thyronine in Development and Regression of Experimental Atherosclerosis in Rabbits ... 

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