Trichoderma reesei growth

Table I. Growth of Trichoderma reesei QM9414 (Tr) and T. harzianum IMI275950 (Th) on wheat straw lignocellulose (lignocell.) or derived cellulosic materials from straw (cell., cellulose hemicell-A, hemicellulose-A holocell., holocellulose)...

It has been proposed that the production of xylanases and cellulases is under separate regulatory control in some filamentous fungi (1). Hrmova et al. (42) reached a similar conclusion after monitoring the daily production of these enzymes in Trichoderma reesei QM 9414. Xylanase and cellulase activities followed independent production profiles during fungal growth. The same effect has been observed in batch cultures of T. harzianum. We have observed peak xylanase activity on the third day of growth whereas the cellulase activity peaked after day five or six (unpublished). 

Tuerker and Mavituna immobilized Trichoderma reesei within the open porous networks of reticulated polyurethane foam matrices. Growth pattern, glucose consumption, and cellulase production were compared with those of freely suspended cells. The method of immobilization was simple and had no detrimental effect on cell activity. Hundreds of similar projects could be cited. Not all rated the use of polymethane as the preferred technique. If a statistical analysis were conducted on aU the immobilization literature, we are sure that no single technique would be dominant. However, the combination of ease of immobilization, cost of materials, flow-through properties, control of flux rate through the immobilizing membrane, high surface-to-volume ratio, and other factors make polymethane a viable substratum for the continuous production of proteins. 

Index Entries Trichoderma reesei fermentation cellulase growth characterization cellulose hydrolysis. 

Pakula TM, Salonen K, Uusitalo J, Penttila M (2005) The effect of specific growth rate on protein synthesis and secretion in the filamentous fungus Trichoderma reesei. Microbiology... 

Only one study (Toma et al., 1991) has reported growth and metabolic inhibition in Brevibacterium flavum, S. cerevisiae, and Trichoderma reesei due to... 

Lejeune, R., Baron, G. V. Effect of agitation on growth and enzyme-production of Trichoderma reesei in batch fermentation. Appl Microbiol Biotechnol. 1995, 43, 249-258. 

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