Trichoderma glucosidase

Figure 1. Fractionation of proteins in the culture filtrate of Trichoderma reesei according to their pi values Xyl, xylanase Ara, arabinosidase AE, acetyl esterase / X, /3-xylosidase aG, a-glucuronidase / G, / -glucosidase CBH, cellobiohydrolase EG, endoglucanase. Chromatofocusing was performed in a PBE-94 anion exchange resin (Pharmacia) with a pH-gradient created by ampholyte buffers (Pharmacia). Solid line, A dotted line, pH. (Reproduced with permission from ref. 24. Copyright 1988.)...

Evidence for Ex-1 to be an Exo-type Component. The time course of CMC hydrolysis by Ex-1 is shown in Figure 10. The hydrolysis proceeded rapidly at first, but it reached a plateau and seemed to stop after 3 hr. This is characteristic of the hydrolysis by exo-type cellulase, as has been reported for exocellulase of glucosidase type from T. viride (7) and for another Trichoderma exocellulase of Avicelase type (10). 

Index Entries Cellulase production Trichoderma reesei RUT C30 pH profiling p-glucosidase shake flask fermentor. 

Carbohydrase [(Trichoderma longibrachiatum var.) (formerly reesei)] Produced as an off white to tan, amorphous powder or as a liquid by controlled fermentation using Trichoderma longibrachiatum var. Soluble in water (the solution is usually tan to brown), but practically insoluble in alcohol, in chloroform, and in ether. Major active principles (1) cellulose, (2) /3-glucanase, (3) /3-D-glucosidase, (4) hemicellulase, and (5) pentosanase. Typical applications used in the preparation of fruit juices, wine, vegetable oils, beer, and baked goods. 

D-Glucosidase carbohydrase (1) Aspergillus niger var. (2) Trichoderma longibrachiatum (3-D-glucoside glucohydrolase 3.2.1.21... 

Values of the kinetic constants for beta-glucosidase from Trichoderma and Aspergillus are shown in Table 2. 

Nojirimycin B (248, mannojirimycin) was isolated from the culture broth of Streptomyces lavendulae, along with nojirimycin [550]. The structure of 248 was established by NMR and by its oxidation to mannonic-6-lactam. Nojirimycin B was a potent inhibitor of apricot emulsin and rat epididymal a-mannosidase, but was less active against Trichoderma viride p-glucosidase. It was weakly active against Xanthomonas oryzae, but inactive against other bacteria [550]. 

A commercial preparation produced by Trichoderma reesei (Spezyme CP) and supplied by Genencor (Rochester, NY, USA) was used as the cellulase enzyme in this study. This preparation was supplemented with a (3-glucosidase preparation (Novozym 188) produced by Aspergillus niger and supplied by Sigma (C6105). Various commercial xylanase preparations were kindly provided by various North American suppliers, and their cellulolytic and hemicellulolytic activities were evaluated (Table 1). BioCat xylanase was in powder form and was suspended in 100 mM sodium acetate buffer (pH 5) at a concentration of 10 mg/ml before use. 

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