Transmission electron microscopy inducing contrast

Kimura, Shirai and coworkers used two chiral dimeric porphyrins 95 and 96 to investigate their self-assembling behavior [162,163]. While incorporation into fibers made of the alkylamide derivatives of (fl,fl)-DACH, 95 formed stable well-resolved fibrous assemblies as visualized by transmission electron microscopy, the fluorescence of which was not quenched by external electron acceptors [162]. However, the induced CD was not detected indicating an inability of 95 to form chirally orientated aggregates under the applied conditions. In contrast, 96 was able to produce optically active inter molecular self-assemblies with an enhanced chiroptical response through the //-oxo bridging in an alkali solution, while intramolecular //-oxo dimer formation was excluded on the basis of steric reasons [163]. 

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