Transgene excision

Marker excision by site-specific recombination Very clean excision, small footprint Complex cloning procedure. Requires additional transgene encoding Cre recombinase 39... 

Jones, J. D. G., Shlumukov, L., Garland, E., English, J., Scofield, S. R., Bishop, G. J. and Harrison, K. 1992. Effective vectors for transformation, expression of heterologous genes, and assaying transposon excision in plants. Transgenic Research, 1 285-297. 

The first subsurface bone tissue Raman spectroscopic measurements were performed using picosecond time-resolved Raman spectroscopy on excised equine cortical bone [56, 57], In these experiments it was shown that a polystyrene backing could be detected through 0.3 mm of bone. The same picosecond technology was used to perform the first transcutaneous Raman spectroscopic measurements of bone tissue [58]. In this study, the cortical bone mineral/matrix ratios of excised limbs of wild type and transgenic (oim/oim) mice were compared and the differences demonstrated. 

Visualize the transgene DNA using long wave UV and excise the band of interest. 

Fig. 16.6. Single cell activation of a Cre-recombinase fused with the ERT receptor upon two-photon release of its caged hgand. The transgenic embryo expresses a GFP gene flanked by two loxP sites. When these are excised, a dsRed gene is expressed in the target cell and its descendents, the red cells, in the eye of this embryo shown at two different magnifications...

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