Transfer reactions 3-glucosidases

Maltase and a-glucosidases in general catalyze transfer reactions in the same manner as galactosidases. It was shown many years ago that maltose hydrolysis could be reversed, although the structure of the product was not established conclusively. Experiments with maltose and labeled glucose have shown the incorporation of the isotope into maltose during incubation with an enzyme preparation from intestinal mucosa.i ... 

Since the beginning of enzyme catalysis in microemulsions in the late 1970s, several biocatalytic transformations of various hydrophilic and hydrophobic substrates have been demonstrated. Examples include reverse hydrolytic reactions such as peptide synthesis [44], synthesis of esters through esterification and transesterification reactions [42,45-48], resolution of racemic amino acids [49], oxidation and reduction of steroids and terpenes [50,51], electron-transfer reactions, [52], production of hydrogen [53], and synthesis of phenolic and aromatic amine polymers [54]. Isolated enzymes including various hydrolytic enzymes (proteases, lipases, esterases, glucosidases), oxidoreductases, as well as multienzyme systems [52], were anployed. 

That the same enzyme might catalyze transfer and hydrolytic reactions was suspected early and ultimately shown (16) for levansucrase however, the first clear demonstrations of this capacity in 1950 involved two well known hydrolases, yeast invertase (17, 18) and / -glucosidase (19). 

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