Transfer Reactions Catalyzed by Glycosidases

D being a glycosyl group. The reaction occurs in two main steps, formation of a glycosyl enzyme, and transfer of the glycosyl group to a water molecule. 

Other hydroxylated derivatives (A—OH), such as alcohols or sugars, are also possible acceptors  

There are presumably two configurational inversions in such a mechanism, so that the anomeric configuration of the newly formed glycosidic bond is the same as that of the donor. 

Soluble enzymes, unless stated otherwise. Enzyme immobilized on tresyl-activated Sepharose. 

---