Transfer Gas Chromatography- TLC

Many liquids and solids, issuing in gaseous form, can be spotted directly on to an adsorbent layer. Prior condensation in a cooled tube and subsequent extraction are thus circumvented. Details of this useful combination can be found in Chapter F (p. 114). 

As long ago as 1946, Consden, Gordon and Martin [137] carried out electrophoretic separation of a mixture of amino acids and peptides on a thin layer of silica gel. Although this technique was accompanied by no particular difficulties, it could not at first keep up with the development at the same time of paper electrophoresis, which was so extremely simple to carry out [129, 268, 777]. Only in recent years, after Stahl in fundamental work had shown how to prepare and use thin layers of adsorbent, has the idea of thin-layer electrophoresis been taken up again. It has the advantage over paper electrophoresis, that interfering adsorption effects can often be avoided through appropriate choice of adsorbent the scope of the method can thus be markedly broadened. In addition, the more uniform and finer structure of the adsorbent used for thin-layer electrophoresis, compared with filter paper, sometimes leads to better resolution. 

All possible fields of application of thin-layer electrophoresis cannot be reviewed since the method is still too new. Only the essential aspects concerning theory, operation and application of the method are thus given below  

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