Transduction by bacteriophage

The time-of-entry map in Fig. 26-4A is based not only on interrupted matings but also on the use of transduction by bacteriophage Transduction... 

Transduction is the term for the transfer of genes from one bacterium to another by bacteriophage particles. The amount of DNA that can be transferred is limited by the size of the DNA that can be carried within the phage head. The specificity of the bacteriophage for receptors on the host bacterium limits any role of transduction in the transfer of DNA between species of bacteria. However, the protection of the labile DNA molecule by the phage coat... 

McHenney MA, Baltz RH. Transduction of plasmid DNA in Streptomyces spp. and related genera by bacteriophage FP43. J Bacteriol 1988 170 2276-2282. 

Alkaline glutaraldehyde (2%) inhibits the transduction of P. aeruginosa PAO by bacteriophage FI 16, but is less effective in inactivating the phage [165]. Lower concentrations of the aldehyde (0.5%) reduce the titer of MS2-and K-coliphage by a factor of Iff within 20 minutes [166]. 

Transduction DNA fragments are taken up by a bacteriophage and transferred to a second organism when the phage infects it. 

The transfer of resistance can be achieved by conjugation, transduction, and transformation. There is also a phenomenon of transposition by which resistance determinants pass from one plasmid to another or to a chromosome or to a bacteriophage, thus allowing construction of new plasmids under the pressure of new antibiotic exposure. 

Finally, in transduction, the transfer of recombinant non-viral DNA to a cell is achieved by a virus. This is the method of choice for the introduction of recombinant X bacteriophages and cosmids into E. coli cells. 

Transduction The transfer of genetic material from one bacterium to another by a bacteriophage. 

Transduction— transfer of genetic material or characteristics from one bacterial cell to another by the incorporation of bacterial DNA into a bacteriophage... 

In other bacteria genetic material is transferred by transduction. In this process part of a bacterial chromosome is transported (transduced) by a bacteriophage (virus cf. Section 8) into the receptor bacterium. Bacterial genetics has become a very valuable tool for many fundamental questions in genetics. 

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