Transcription and Translation in a Cell-Free System

The final demonstration that a biochemical reaction proceeds as supposed should be provided, if possible, by experiments in a cell-free system. Although such a cell-free system does not contain any intact cells, in many cases it does contain certain organelles, for example, chloroplasts or ribosomes. In addition, all necessary enzymes and cofactors are present. Compared with the living cell, cell-free systems that still contain organelles are very simple and are suitable for the elucidation of still unanswered questions. 

After a certain time of incubation the cell-free system was examined to ascertain whether protein had been made (Table 3). This proved to be 

Matrix (from pea) Total soluble protein Pea globulin tein after subtraction of the blank of 0.13 

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Control of Character Formation by Nucleic Acids 7. Transcription and Translation in a Cell-free System... 

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