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Transamination coupled

A key step in the synthesis of the spiroketal subunit is the convergent union of intermediates 8 and 9 through an Evans asymmetric aldol reaction (see Scheme 2). Coupling of aldehyde 9 with the boron enolate derived from imide 8 through an asymmetric aldol condensation is followed by transamination with an excess of aluminum amide reagent to afford intermediate 38 in an overall yield of 85 % (see Scheme 7). During the course of the asymmetric aldol condensation... [Pg.496]

DNA and RNA may be modified with hydrazide-reactive probes by reacting their cytosine residues with bisulfite to form reactive sulfone intermediates. These derivatives undergo transamination to couple hydrazide- or amine-containing probes (Draper and Gold, 1980) (Chapter 27, Section 2.1). [Pg.412]

For example, coupling alanine transamination (via ALT) with GLDH is shown in Figure 6.6b. A similar scheme can be drawn using, for example, aspartate transaminase in place of alanine transaminase. [Pg.178]

From a practical viewpoint they suffer from serious product inhibition and/or equilibrium constraints. One way of avoiding the latter issue is to couple the transamination step to a second enzymatic step which removes the coproduct In the example shown in Scheme 6.16 acetophenone undergoes TA-catalyzed transamination using L-alanine as the amine donor. The unfavorable equilibrium and... [Pg.119]

The first method in this application, however, was limited to modifying alcohol-containing colorants onto polymers containing grafted succinic anhydride transesterification, and transaminated reactions were also reported. In the second method water-soluble colorants were prepared by azo coupling of colorants with alkoxyether functionalized aniline. [Pg.4]

Since the use of ammonia is not practical in transition metal catalyzed processes, the identification of its synthetic equivalents is of major importance. Benzophenone imine was found to couple with 3-bromopyridine readily under the above mentioned conditions (7.72.), The masking benzophenone was removed in transamination with hydroxylamine, which gave the desired 3-aminopyridine in 81% overall yield.92 Allylamine was also successfully employed as ammonia equivalent93... [Pg.165]

Biotin acts as a carboxyl group carrier in a series of carboxylation reactions, a function originally suggested by the fact that aspartate partially replaces biotin in promoting the growth of the yeast Torula cremonis. Aspartate was known to arise by transamination from oxaloacetate, which in turn could be formed by carboxylation of pyruvate. Subsequent studies showed that biotin was needed for an enzymatic ATP-dependent reaction of pyruvate with bicarbonate ion to form oxaloacetate (Eq. 14-3). This is a (3 carboxylation coupled to the hydrolysis of ATP. [Pg.724]

Answer The measurement of the activity of alanine aminotransferase by measurement of the reaction of its product with lactate dehydrogenase is an example of a coupled assay. The product of the transamination (pyruvate) is rapidly consumed in the subsequent indicator reaction, catalyzed by an excess of lactate dehydrogenase. The dehydrogenase uses the cofactor NADH, the disappearance of which is conveniently measured by observing the rate of decrease in NADH absorption at 340 nm. Thus, the rate of disappearance of NADH is a measure of the rate of the aminotransferase reaction, if NADH and lactate dehydrogenase are added in excess. [Pg.198]

The assay used to quantify blood urea nitrogen relies on coupling the production of ammonia and carbon dioxide from urea to the transamination of a-ketoglutarate to glutamate and the subsequent oxidation of NADH ( 340) (Fig. 16-2). [Pg.254]

Giuseppone N, Schmitt J-L, Schwartz E, Lehn J-M (2005) Transaminations under scandium triflate catalysis. Independent and constitutionally coupled reactions. J Am Chem Soc 127 5528-5539... [Pg.50]

Benzophenone imine may be used as an ammonia equivalent with halopyridines as well. The coupHng of the bromopyridine below, followed by deprotection via transamination with hydroxylamine, proceeded in 81% yield over the two steps, Eq. (158) [ 128]. Analogously, Puttman found that allylamine could also be used as an ammonia equivalent in the C-N coupling reactions of halopyridines [114]. [Pg.190]

To give a real example, have a closer look on main functions and cycle of magnesium in green plants. Control on autocatalysis depends on the principal functions of Mg, that is, on photosynthesis when substantial parts of Mg taken up by roots are allocated to chlorophyll and rubisco synthesis, less will be available for other metabolic pathways, reducing the turnovers there unless there are lots of Mg around like in marine plants. In addition, the tricarboxylate cycle (citrate cycle) requires Mg (besides Fe and Mn) to produce the enzymes hence some Mg (as well as Fe, Mn) must be invested to produce the citrate (malate, oxaloacetate (aspartate)) ions delivered by the roots to render Mg (and other metals) in turn bioavailable by means of complexation and resorption of almost neutral complex entities. Furthermore, the tricarboxylate cycle is coupled to biosynthesis of amino acids by redox transamination hence there will be both competition at the metal center(s) and possible extraction of metal ions from enzymes once NHj and electrons are... [Pg.111]


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