Total genome amplification

Total genome amplification method One approach to PCR sampling of an entire genome is the method of primer extension preamplification (PEP). A mixture of all possible 4" primers of length n is generated by automated oligonucleotide synthesis, using at... 

Amplified fragment length polymorphism (AFLP), developed by Vos et al. [453], is also a new technique for DNA fingerprinting. This technique is based on the selective amplification of restriction fragments (SARE) by PCR from a digest of total genomic DNA. 

Kenzelmann M, Klaren R, Hergenhahn M et al. High-accuracy amplification of nanogram total RNA amounts for gene profiling. Genomics 2004 83 550-558. 

How an extract was produced from a sample and prepared for hybridization, e. g., extraction method, whether total RNA, mRNA, or genomic DNA was extracted, exogenous sequences (spikes) added (for each spike added type, name/clone id, quantity added), target amplification (RNA polymerases, PCR). 

The genomic DNA amplification of horn fly populations using the 16 RAPD selected primers produced 321 fragments. Tliese fragments varied from 1714 to 229 bp. The total number of bands produced by each primer varied from 10 (primer H20) to 28 (primer G4 and G16). From the 16 selected primers, 12 generated 15 bands or more. Regarding the total number of bands produced by population, the most polymorphic was RN (70 bands), followed by RJ (68 bands), MS and RS (62 bands), and RR (59 bands) (Table 12.2). 

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