TOSC assay

TOSC Assay (Total Oxyradical Scavenging Capacity)... 

This assay has been used to determine the antioxidant activity of common vegetables, fruits including apples and strawberries (Eberhardt and others 2000 Chu and others 2002 Sun and others 2002 MacLean and others 2003 Meyers and others 2003), and fruit juices (Lichtenthaler and Marx 2005). No studies have yet reported the application of TOSC assay for the assessment of in vivo antioxidant status. 

TOSC assay KMBA ethylene ABAP Gas chromatography (area under curve) W12... 

The total oxygen-scavenging capacity (TOSC) assay is based on the oxidation of a-keto-y -methiolbutyric acid (KMBA) to ethylene. Ethylene formation is monitored by gas chromatography in the course of reaction and areas below the kinetic curves for control sample and analyzed sample are compared. The oxidant is usually ABAP, but other oxidants were also used and compared (R5, W12). 

Davis BM, Senthilmohan ST, McEwan MJ. Direct determination of antixoidants in whole olive oil using the SIFT-MS-TOSC assay. J Am Oil Chem Soc. 2011 88 785-92. 

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