Tomato bushy stunt virus, TBSV

TBSV. See Tomato bushy stunt virus (TBSV) TCV. See Turnip crinkle virus (TCV)... 

C-terminal interactions and, 267-268 X-ray analysis, of virus structure, 1, 3, 221 X-ray crystallography, 93-94, 221, 385-386 cryoelectron microscopy with, 54—56 of southern bean mosaic virus (SBMV), 39 of tomato bushy stunt virus (TBSV), 39 X-ray detectors, 40-41... 

Donald L. D. Caspar showed that a particular virus, tomato bushy stunt virus (TBSV), has a capsid structure with icosahedral symmetry. 

Figure 2. Two-dimensional agarose gel electrophoresis of car-boxylated latex spheres. The following mixture was made 24 pi of 2.5% carboxylated latex spheres with an R of 30 nm (purchased from Polysciences, Inc.) 6.0 pi containing 4 pg each of bacteriophages T3 and (j)II (R = 30.1 nm), 2 pg of bacteriophage P22 (R = 31.4 nm) 1.0 pi containing 3 pg of tomato bushy stunt virus (TBSV R = 17.0 nm) (20) and 28 pi of 6% sucrose, 400 pg/ml bromphenol blue in 0.025 M sodium phosphate, pH 7.4,...

In collaboration with the Virology Laboratory of the Institut de Biologie Moleculaire et Cellulaire in Strasbourg, the Laboratory of Molecular Acoustics has studied the ultrasonic absorption of two small icosahedral viruses, bromegrass mosaic virus (BMV) and tomato bushy stunt virus (TBSV). Measurements carried out between 0.6 and 40 MHz showed that ultrasonic waves in the MHz range are absorbed much more by capsids than by dissociated protein. Examples of ultrasonic spectra of solutions of BMV protein capsids and of subunit-dimers are shown in Fig. 1 (at 5 mg/ml in 10 mM Na cacodylate, 1.00 M NaCl, at 23°C capsids , pH=4.80 dimers o, pH = 6.6o). Spectra obtained for BMV (A, pH = 5 00, at the same particle concentration as for the BMV capsids, otherwise similar conditions) and for the pure solvent ( — ) are also shown. 

Several plant vims coat proteins, including those of TMV, CPMV, AlMV and Tomato bushy stunt vims (TBSV), have been used to produce and deliver antigenic determinants from a variety of viral and bacterial pathogens. These data have been summarized in numerous publications and several reviews [12,13]. The ease of virus purification coupled with enhanced peptide immunogenicity when fused to carrier molecules makes this approach very attractive for vaccine development. 

Several plant viruses have been studied using X-ray crystallography and conventional X-ray sources. These are, in no particular order, TBSV (tomato bushy stunt virus), SBMV (southern bean mosaic virus) and STN V (satellite tobacco necrosis virus) - all spherical viruses - and TMV (tobacco mosaic virus) - a cylindrical virus. These virus crystals diffract relatively well and are reasonably stable to radiation. 

The tertiary fold of three of the rhinovirus proteins (VP1, VP2 and VP3) and their quaternary organisation within the HRV14 capsid are similar to the structures of the plant viruses TBSV (tomato bushy stunt virus) and SBMV (southern bean mosaic virus), agreeing to within approximately 3 A. In HRV14 VP4 is an internal structural protein in contact with VP1 and VP2. Protrusions on VP1, VP2 and VP3 together create a deep cleft or canyon on the viral surface which is 25 A deep and... 

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