Tobacco ringspot virus

Fig. 4. The self-cleaving hairpin motif from the satellite RNA of tobacco ringspot virus (sTobRV). The arrow indicates the cleavage site. The numbers in brackets indicate the nucleotide positions within the sTobRV satellite RNA...

Figure 12-28 (A) A hairpin ribozyme formed from the minus strand of a satellite RNA associated with tobacco ringspot virus. On the basis of hydroxyl radical footprinting (see Fig. 5-50), to identify protected areas a folding pattern that brings domains A and B together to form a compact catalytic core has been proposed.798 (B) A "leadzyme," a ribozyme dependent upon Pb2+ for cleavage of RNA.802 803 (C) An RNA-cleaving DNA enzyme.804...

Buzayan, J.M., Hampel, A. and Bruening, G. (1986) Nucleotide sequence and newly formed phosphodiester bond of spontaneously ligated satellite tobacco ringspot virus RNA. Nucleic Acids Res., 14, 9729-9743. 

Wolfson, A., Kisseleva, N., Khvorova, A., Westhof, E., and Schiemann, O. (2008). Correlation of the fast cleavage behaviour of the minimal and tertiary stabilized hammerhead ribozymes derived from Tobacco Ringspot Virus RNA with EPR data. Oligonucleotides 18, 101-110. 

The conventional hairpin ribozyme is derived from the minus strand of Tobacco ringspot virus satellite RNA [12a,b]. It works in cis (intramolecularly) in nature, but... 

Tobacco ringspot virus (TRSV) causes a disease in soybean called bud blight and may be a contributor to greenbean syndrome (Demski Kuhn, revised by Hartman, 1999). TRSV is a nepovirus that the nematode Xiphimena americanum can transmit, but it is also seedborne. The RNA genome of the virus has two parts, each contained... 

Tobacco blue mold tobacco mosaic tobacco ringspot black shank black root rot Peronospora tabacina 5 virus virus Phytophthora parasitica Thielaviopsis basicola, 31,32 1.3 1.3 33 34... 

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