Tobacco mosaic virus electrophoresis

Sumner s analytical studies convinced him that urease was a protein. This conclusion was resisted by the chemical community but John H. Northrop s (1891-1987) crystallization of pepsin in 1930 at the Rockefeller Institute in New York City and its unambiguous decomposition into amino acids fully vindicated Sumner. Sumner and Northrop were able to make use of the ultracentrifiige developed by Svedberg and the electrophoresis technique developed by his student Tiselius to fully establish purities and molecular weights of their enzymes. Sumner and his coworkers then crystallized trypsin and chymotrypsin. Sumner and Northrop shared the 1946 Nobel Prize in chemistry with Wendell M. Stanley (1904—71), who in 1935 crystallized the tobacco mosaic virus in his laboratory at the Rockefeller Institute. 

Furthermore, Grossman and Soane [66] have studied the effect of molecular orientation on the electrophoretic mobility of a macro ion in free solution (the tobacco mosaic virus was a model) by using the capillary zone electrophoresis technique. 

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