Tobacco leaf disks

The influence of Ag+ and CO on ethylene action was observed in senescing tobacco leaf disks in which loss of chlorophyll was taken as an index of senescence (Fig. 7). 

Figure 7. Influence of Ag (10 ppm), C02 (10% ), and AVG (O.lmM) on chlorophyll retention in aging tobacco leaf disks senesing in the dark for six days...

Anderson J D et al 1982 Induction of ethylene biosynthesis in tobacco leaf-disks by cell-wall digesting enzymes. Biochem/Biophysiol Rev 107 588-596... 

Adedipe et a// found that Bel W, tobacco leaves were more sensitive to ozone when attached, rather than detached or used as leaf disks. Effects were seen as visual injury and change in chlorophyll content. 

Leaf disk test A caterpillar of the tobacco homworm faces a choice of four leaf disks in a Petri dish. The leaf disks are treated with different concentrations of tannic acid ... 

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