Titration curve of protein

Bashford, D., Karplus, M. Multiple-site titration curves of proteins an analysis of exact and approximate methods for their calculation. J. Phys. Chem. 95 (1991) 9556-9561. 

For further discussion of experimental methods for determination of electrophoretic titration curves of proteins, see the recent study by Gianazza et al. [129], For discussion of the free solution mobility of DNA see Stellwagen et al. [368],... 

The Interpretation of Hydrogen Ion Titration Curves of Proteins Charles Tanford... 

Tanford, C. 1962. The interpretation of hydrogen ion titration curves of proteins. In Advances in Protein Chemistry, Vol. 17, C. B. Anfinsen, K. Bailey and J. T. Edsall (Editors). Academic Press, New York. 

Make a table of characteristic pKa values for acidic and basic groups in proteins. Which of these groups contribute most significantly to the titration curves of proteins ... 

Electrostatic theory has also been used successfully to interpret titration curves of proteins in which the net negative or positive charge distributed over the surface of the protein varies continuously from high pH to low as more protons are added.19... 

Hydrogen ion titration curves of proteins provide a powerful tool to reveal many aspects of the structures of individual proteins. The characteristic ionization constants of the acidic and basic groups in the amino acids and peptides may be profoundly modified when these groups are incorporated in a protein molecule. An increasing number of proteins have been found in which potentially reactive groups are inaccessible for titration in the native molecule, and become available only after denaturation. Such findings can, in the years ahead, be correlated with detailed knowledge of the three dimensional structure of proteins, as obtained by X-ray diffraction and other methods. The present state of the field is reviewed by Tanford, who has done so much to advance it over the last decade. 

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