Titin differential splicing

B. Differential Splicing of Titin Exon Shuffling Creates Functionally... 

Segment contains tandem Ig repeats and PEVK repeats, both acting as molecular spring elements. This segment is extensively differentially spliced. In the shortest cardiac-specific N2B titin isoform (linked to inclusion of exon 49), exon 50 is spliced direcdy to exon 225. In more compliant cardiac and skeletal titin isoforms, exon 50 is spliced to exon 51 (linked to additional inclusion of exons in the segment 52-224). 

Similarly, multiple genes, alternative RNA splicing, and posttranslational modifications result in multiple essential and regulatory light chains, tropomyosins, titins, and other myofibrillar proteins. Energy pathway enzymes are differentially expressed in various skeletal fiber types, in cardiac and smooth muscle, and at different stages of development. This also applies to Ca regulatory proteins such as the... 

Titin is expressed in embryonic and adult cardiac and skeletal muscle. Isoforms with different Mr values are expressed differentially during embryonic development. Evidence suggests that there is a single gene in domestic fowl and that the isoforms are generated by alternate splicing (Tan et al., 1993). 

---