Tissue plasminogen activator purification

Fig. 2. Outline of possible steps in the recovery and purification sequence for recombinant tissue plasminogen activator derived from recombinant CHO...

Fig. 3. Overview of purification sequence for the nonrecombinant tissue plasminogen activator (t-PA) which also contains urokinase plasminogen activator (u-PA). Serum-free culture conditional media is from normal human cell line. The temperature for all steps, except for size-exclusion chromatography...

Denman J, Hayes M, O Day C, et al. Transgenic expression of a variant of human tissue-type plasminogen activator in goat milk purification and characterization of the recombinant enzyme. Biotechnology (NY), 1991 9(9) 839-843. 

Hostettmann K, Marston A, Hostettmann M (1998) Preparative chromatography techniques applications in natural product isolation. Springer, Berlin, Heidelberg, New York, 244 pp Hua ZC (1997) Renaturation and purification of recombinant tissue-type plasminogen activator expressed in E. coli. lUBMB Life 41(4) 815-820 Huang RB, Andrews BA, Asenjo JA (1991) Diflerential product release (DPR) of proteins from yeast a new technique for selective product recovery from microbial cells. Biotechnol Bioeng 38 977-985... 

Dodd I., Jalalpour S., Southwick W., Newsome P., Browne M.J. and Robinson J.H. 1986. Large scale, rapid purification of recombinant tissue-type plasminogen activator, Febs Lett, 209, 13. 

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