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TIR-FLIM

The upgrade of a frequency-domain fluorescence lifetime imaging microscope (FLIM) to a prismless objective-based total internal reflection-FLIM (TIR-FLIM) system is described. By off-axis coupling of the intensity-modulated laser from a fiber and using a high numerical aperture oil objective, TIR-FLIM can be readily achieved. The usefulness of the technique is demonstrated by a fluorescence resonance energy transfer study of Annexin A4 relocation and two-dimensional crystal formation near the plasma membrane of cultured mammalian cells. Possible future applications and comparison to other techniques are discussed. [Pg.405]

Upgrading wide-field FLIM to a TIR-FLIM microscope... [Pg.411]

See other pages where TIR-FLIM is mentioned: [Pg.409]    [Pg.413]    [Pg.414]    [Pg.416]    [Pg.418]    [Pg.419]    [Pg.420]    [Pg.409]    [Pg.413]    [Pg.414]    [Pg.416]    [Pg.418]    [Pg.419]    [Pg.420]    [Pg.413]    [Pg.415]    [Pg.154]   


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