TIM protein

J. C. Leloup and A. Goldbeter, A model for circadian rhythms in Drosophila incorporating the formation of a complex between the PER and TIM proteins. J. Biol. Rhythms 13, 70-87 (1998). 

Roshash In the 10 years that this has been described, no one has seen PER or TIM protein or mRNA expression outside of these five centres. When you use pergald or timgali drivers, it is indeed present elsewhere. 

Koehler CM (2004b) The small Tim proteins and the twin Cx3C motif. Trends Biochem Sci 29 1-4... 

Cryptochrome genes have been found in many organisms. In the fly Drosophila cryptochrome appears to interact directly with the clock proteins that control the circadian cycle. Most important are products of two genes per (period) and tim (timeless). They are helix-loop-helix DNA binding proteins that form heterodimers, are translocated to the nucleus, and repress their own transcription. Morning light leads to a rapid disappearance of e TIM protein. The cryptochrome CRY appears to react directly with TIM to inactivate it. However, details remain to be learned. " The circadian clock mechanism appears to be universal and the cryptochrome-2 mcryl gene) appears to function in the mouse. A human cDNA clone was found to have a 48% identity with a relative of cryptochromes, the (6-4) photolyase of Drosophila. 

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