Tile assay

Two different field assays were developed by Slattery et al. (1995) to examine microalgal settlement. In the first experiment, Antarctic soft coral extracts were incorporated into a 5% agar solution at dry weight concentrations equivalent to 

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The key goal of this system is to produce periodic (or aperiodic) assays from DNA motifs that can act as tiling elements in two or three dimensions. These tiles must be rigid components, because flexibility can lead to un-... 

The process is carried out as follows A mixture of 20 c.c. of lemon oil, 20 c.c. of 90 per cent, alcohol, 20 c.c. of a 5 per cent, solution of hydroxylamine hydrochloride in 80 per cent, alcohol, and 8 c.c. of normal alcoholic potash solution is gently boiled for thirty minutes under a reflux condenser. A similar mixture, without the lemon oil, is treated in exactly the same manner. After cooling, the condensers are washed down with water to restore any traces of hydroxylamine to their respective flasks, and the contents of the flasks duuted with about 200 c.c. of water. A few drops of phenolphthalein are added to the flasks, and the contents first neutralised with semi-normal alkali. The hydroxylamine is now titrated with semi-normal sulphuric acid solution, using methyl orange as indicator. A few drops of the indicator should be added to the flask, so that a general indication of the approach of the end reaction is given, but the end reaction itself should bie observed by the familiar process of spotting on a white tile, on drops of the indicator. Each cubic centimetre of half-normal acid used in the blank experiment, over that used in the assay experiment, is equivalent to 0 076 grm. of citral. 

While tile secondary assays are being carried out, tiie pure active product is also undergoing structure elucidation. The application of high field NMR technology is particularly important. As the structure elucidation progresses and one is able to identify the specific chemical structure, then some preliminary... 

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