Through dynamic light scattering

The basic technique is only applicable to dilute suspensions where multiple scattering does not occur and this technique is sometimes referred to as through dynamic light scattering. The introduction of the controlled reference method has extended it to more concentrated systems [275]. 

The autocorrelation function of the scattered intensity G t) is defined as the product of the light intensity at the detector at time t and at a short time later t + t. 

The normalized first order autocorrelation function G(r) can be calculated from the measured function  

The ratio B/A is often designated as the intercept, as a percentage merit or as a signal to noise ratio. 

The decay rate Gis linked to the translational diffusion coefficient D by  

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FIGURE 1 Effect of (sequential) extrusion of MLV dispersions through polycarbonate membrane filters (Unipore) with pore sizes of 1.0, 0.6, 0.4, 0.2, and 0.1 ym on the mean liposome diameter. DXR-containing MLV (phosphatidylcholine/phosphatidylserine/ cholesterol 10 1 4) mean diameter of nonextruded dispersion about 2 ym pH 4. Mean particle size determined by dynamic Light scattering (Nanosizer, Coulter Electronics). (From Crommelin and Storm, 1987.)... 

Equation (22) has been confirmed by a variety of techniques including neutron scattering, dynamic light scattering, and osmotic pressured measurements [23]. As concentration increases the concentration blob decreases in size until the Kuhn length is reached and the coil displays concentrated or melt Gaussian structure. The coil accommodates concentrations between the overlap and concentrated through adjustment of the concentration blob size. 

In contrast, in dynamic light scattering (DLS) the temporal variation of the intensity is measured and is represented usually through what is known as the intensity autocorrelation function. The diffusion coefficients of the particles, particle size, and size distribution can be deduced from such measurements. There are many variations of dynamic light scattering, and... 

Standard static and dynamic light scattering methods assume that there is very little multiple scattering by the particles, that is, the dispersion has to be sufficiently dilute so that the photons are scattered only once as they pass through the sample. Is there a way to look inside a dispersion that is cloudy or milky, such as a foam, and to extract information on the local structure and its kinetics and relaxation Or, is it possible to tailor a dispersion so that... 

The discussion of dynamic light scattering (DLS) through Section II.A. 1 demonstrates that the field ACF reduces to [Eq. (33)]... 

Wojtyk, J., McKerrow, A., Kazmaier, R and Buncel, E. (1999). Quantitative investigations of the aggregation behavior of hydrophobic anilino squaraine dyes through UV/vis spectroscopy and dynamic light scattering. Can. J. Chem.—Rev. Can. Chem., 77, 903-12. [88, 205]... 

Dynamic light scattering (DLS) measurements were performed on a DynaPro-801 instrument (Protein Solutions) and the data analyzed with the Autopro software package. All proteins were filtered through a 0.10 micron syringe filter and analyzed at 22°C. The translational difilisional coefficient (Dr) was obtained directly and the hydrodynamic radius (Rh) is derived by a rearrangement of the following equation ... 

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