Three-dimension cell culture

Since dielectrophoresis relies on the geometry of the electrodes to create the nonuniform electric fields, the method has been limited to the creation of 2D cell patterns within 3D culture constructs (Figure 32.5c). True 3D cell patterning would require more complex electrode geometries than those employed to date. Although dielectrophoresis has been used to levitate cells and could be used for 3D patterning, the method has not been utilized solely or in combination with any other methods to pattern cells in all three dimensions within a 3D culture construct. 

Another option is bioresorbable sUk. The silk matrix is an appropriate three-dimension culture environment for cell attachment and spreading after being processed to extract the allergen component, sericin. The development of twisted fiber architecture gives the scaffold excellent... 

Figure 10.323 Amino acid analysis of three cell culture medium compositions using HPAE-PAD. Separator column AminoPac PA10 column dimensions 250mmx2mm i.d. column temperature 30 °C eluent (A) lOmmol/L NaOH, (B) 250mmol/L NaOH, and (Q 1 mol/L NaOAc in 25 mmol/L NaOH gradient 95.83% A+4.17% B (v/v) from 0 to 2min, to 70.83% A+29.17% B (vAr) in 10 min, iso-craticfor4min,to41.5% A + 185% B+40%C (v/v/v) in 8 min, to 50% A+10% B+40% C (v/ v/v) in 3min, and then to 21.88% A + 8.12%...

This culture can be used to study cells that adhere three-dimension-ally with fibrous collagen in the body, thus reproducing typical body conditions. In reality, in vivo studies smooth muscle cells [5], vein-like cavity formation of endothelial cells of fine blood veins [6], and secretion... 

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