Thread-induced venous thrombosis

Compared to the arterial system, it seems to be more difficult to develop a thrombosis model in venous 

Thrombus formation is induced by inserting the thrombosis catheter into the caval vein via the V. iliaca (7 cm). Then the copper wire is pushed forward 3 cm to liberate the cotton threads into the vessel lumen. At 150 min after thrombus initiation, the caval segment containing the cotton threads and the developed thrombus will be removed, longitudinally opened and the content blotted on filter paper. After weighing the cotton thread with the thrombus, the net thread weight will be subtracted to determine the corrected thrombus weight. 

Corrected thrombus weight after blotting the thrombus on filter paper and subtraction of the net weight of the cotton thread 

The composition of the cotton threaded thrombus shows a composition of fibrin together with tightly aggregated and distorted erythrocytes, thus being in accordance with human deep vein thrombosis structure. Non-occlusive thrombus formation has been successfully inhibited by heparins, prothrombinase complex inhibitors and thrombin inhibitors (Hollenbach et al. 1994,1995). 

In addition to the originally described method, it is possible to measure blood flow by means of an ultrasonic flow probe, attached distally to the position of the cotton threads on the vein. 

---