Third-day Experiments

Students should be able to describe the theory of Michaelis-Menten before entering the lab. 

The instructor should give the students two stock solutions of p-nitrophenyl-/ -D-galactoside (1 and 10 mM). Students should then prepare the following heteroside concentrations in a final volume of 2 ml 0.1, 0.25, 0.5, 0.75,1,2.5, 5,7.5, and 10 mM. 

Students can repeat the last experiment in the presence of two different inhibitor concentrations to determine its effect on the hydrolysis rate. 

The instructor should give students three stock solutions of inhibitor 5, 10, and 30 mM. Students are going to prepare two series of six test tubes, containing each of the following 

The plots obtained correspond to a competitive inhibition characterized by the following equation  

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