Thiram structure

Fig. 10.4 Supposed molecular structures corresponding to the degradation products of thirame by yeast...

Dithiocarbamates can be quantitatively converted to carbon disulfide by reaction with tin(II)chloride in aqueous HCl (1 1) in a closed bottle at 80 C. The CS2 gas produced is absorbed into iso-octane and measured by GC-MS. The analysis of DTCs for this application follows the acid-hydrolysis method using SnCl2/HCl (Reynolds, 2006). For method validation of the DTC pesticides, Thiram (99.5% purity) was used as representative DTC compound considering its simple structure (1 mol of Thiram = 2 mol of CS2 = >1 mg of Thiram theoretically generates 0.6333 mg CS2, 1 mL of 100 ppm Thiram in 25 g of grapes = 2.5 ppm of CS2), see Figure 4.38. The residues were estimated by analysis of CS2 as the DTC hydrolysis products by GC-MS. 

For method validation Thiram (99.5% purity) was used as representative DTC compound considering its simple structure (1 mol of Thiram = 2 mol of C 2)-... 

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