Thioester Transcriptional activator

There are fewer catalytic ribozymes compared to deoxyribozymes. Examples include a trara-splicing ribozyme, an alcohol dehydrogenase, a ligase capable of functioning at low temperature, " a ribozyme that will ligate the 5 -terminus of RNA to a polypeptide, a transcriptional activator and a tRNA aminoacylation catalyst. An RNA aptamer bearing 5 -CoA has been selected to catalyse thioester formation in the presence of biotin-AMP. In vitro selection has also been used to identify allosteric hairpin ribozymes, activated in the presence of short oligonucleotides, and a ribozyme that catalyses amide bond formation from a 2 -amino nucleotide. " ... 

Fukuyama et al. synthesized the alcohol 39 using Evans s chiral auxiliary in the total synthesis of leustroducsin B (37), a potent colony-stimulating factor inducer via NF-kB activation at the transcription level15 (Scheme 2.In). The asymmetric aldol reaction between 38 and the requisite aldehyde proceeded smoothly to afford 39. Protection of the secondary alcohol as the TES ether and removal of the chiral auxiliary with LiSEt furnished the thioester 40. 

---