Thiobarbituric acid test

Nair, V. and Turner, G.A. (1984). The thiobarbituric acid test for lipid peroxidation structure of the adduct with malondi-aldehyde. Lipids 19, 804—805. 

The periodate-thiobarbituric acid test developed by Waravdekar and Saslaw269 is used in the modification of Weissbach and Hur-witz229 for identification of the products of /3-eliminative cleavage. 

Exposure to periodate yields 3-formylpyruvic acid, which reacts with thiobarbituric acid, giving rise to red condensation products having an absorption maximum at 545-550 nm. Certain workers have used the thiobarbituric acid test without previous oxidation of the unsaturated oligo-D-galactosiduronates with periodic acid.3-29,239,260 Even here, the products of reaction with thiobarbituric acid have an absorption maximum at 550 nm. 

The periodic acid-thiobarbituric acid test has some disadvantages that may cause severe errors in the determination of sialic acid. 

Haase, G. and Dunkley, W. L. 1969A. Ascorbic acid and copper in linoleate oxidation. I. Measurement of oxidation by ultraviolet spectrophotometry and the thiobarbituric acid test. J. Lipid Res. 10, 555-560. 

Hoyland, D.V. and Taylor, A.J. 1991. A review of the methodology of the 2-thiobarbituric acid test. Food Chem. 40 271-291. 

Rhee, K.S. 1978. Minimization of further lipid peroxidation in the distillation 2-thiobarbituric acid test of fish and meat. J. Food Sci. 43 1776-1778, 1781. 

Tarladgis, B.G., Pearson, A.M., and Dugan, L.R. Jr. 1964. Chemistry of the 2-thiobarbituric acid test for determination of oxidative rancidity in foods. II.-Formation of the TBA-malonaldehyde complex without acid-heat treatment. J. Sci. Food Agric. 15 602-607. 

Peroxide value, anisidine value and thiobarbituric acid test... 

Asakawa, T. and Matsushita, S. (1979) Thiobarbituric acid test for detecting lipid peroxides, Lipids 14, 401-406. 

The thiobarbituric acid test (AOCS method Cd 19-90), developed initially for measurement of malondialdehyde, is used to measure the oxidative degradation product of polyunsaturated fatty acids (47, 48). However, it lacks sensitivity (49). 

Brett R, Rumsby MG. Susceptibility of myelin gjycerophospholipids and sphingolipids to oxidative attack by hydroxyl free radicals as measured by the thiobarbituric acid test. Neurochem Int 1994 24 241-51. 

Bieri (1959) was able to demonstrate by the thiobarbituric acid test that peroxidation occurred in homogenates of chicken brain incubated in air at 37 °C for 1 hour. The degree of peroxidation in brain homogenates was much higher than in homogenates of liver and muscle moreover, the peroxidation in brain homogenate was independent of whether the chicks had received vitamin E or not. These results indicate that tocopherol does not easily pass the blood-brain barrier. 

According to Zalkin and Tappel (1960) the in vivo peroxidation occurring in vitamin E deficiency damages the mitochondria in the tissues of rats, rabbits, and chicks as measured by oxygen absorption, thiobarbituric acid test, and enzyme inactivation. 

Patton, S. Malonaldehyde, lipid oxidation, and the thiobarbituric acid test. J. Am. Oil Chem. Soc. 51, 114(1974). 

The thiobarbituric acid test (TBA) is a preferred method for detecting lipid peroxidation in biological systems. However, the reaction is nonspecific since a number of primary and secondary products of lipid peroxidation form malonaldehyde which in turn reacts in the TBA test. In food containing oleic and linoleic acids, the TBA-test is not as sensitive as the Fe +-test outlined above. 

The extent of prior oxidative activity in a fat sample may be estimated by the following peroxide value (PV), thiobarbituric acid test (TEA) (which measures the presence of malonaldehyde), anisidine value—Totox, Kreis test, oxirane test, total and volatile carbonyl... 

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