2-Thiobarbituric acid reactive material

Rowley, A., Gutteridge, J.M.C., Blake, D.R, Farr, M. and Halliwell, B. (1984). Lipid peroxidation in rheumatoid arthritis thiobarbituric acid reactive material and catalytic iron salts in synovial fluid from rheumatoid patients. Clin. Sci. 66, 691-695. 

Attack of hydroxyl radical on the material under investigation should not produce thiobarbituric acid-reactive material hence a control is performed in which deoxyribose is omitted from the reaction mixture. 

Fig. 5. Inactivation of Cu,Zn-SOD by glycation reaction in vitro. Purified Cu,Zn-SOD from human erythrocytes was incubated with 10-20 mAf glucose at 4°C under sterile conditions. At appropriate time intervals the SOD activity and thiobarbituric acid-reactive materials were measured.

Shelf Storage Test The test material is stored under similar conditions as in retail and is evaluated for the effectiveness of antioxidants in prolonging the premium quality of the product. Periodic evaluation of the hpid oxidation products (primary or secondary) by chemical tests (e.g., peroxide value, conjugated diene value, 2-thiobarbituric acid reactive substances, hexanal content) or sensory evaluation will be used to find out the onset of oxidation. The main drawback of this kind of evaluation is the time taken therefore, rapid evaluation or accelerated methods are often preferred (19, 51). 

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