Thin-layer chromatography ascorbic acid

DETERMINATION OF ASCORBIC ACID IN POLYVITAMIN PREPARATIONS BY ION-PAIR THIN LAYER CHROMATOGRAPHY... 

The new way of quantitative determination of the ascorbic acid (AC) by means of ion-pair thin layer chromatography (TLC) in organo-aqueous mobile phases containing cetyltrimethylammonium bromide (CTAB) has been alaborated. 

Illner, E. (1984). Separation of ascorbic and isoascorbic acids by thin-layer chromatography on Silufol-ready-made plates. Pharmazie 39 864. 

Thin-Layer Chromatography. - Diacetone ketogulonic acid (2,3 4,6-di-0-isopropylidene-a-L-xy/o-hex-2-ulosonic acid), an intermediate in ascorbic acid manufacture, could be determined in aqueous samples by concentration on to a polymeric Cig adsorbent and separation by ID- or 2D-h.p.t.l.c. ... 

Paper and thin-layer chromatography (TLC) was applied in early analytical studies to increase selectivity in the analysis of ascorbic acid and other similar chemical compounds. Nowadays these methods have little if any qualitative or quantitative use. However, TLC has proven to have some advantages in the analysis of vitamin C compounds, especially in pharmaceuticals, which often consist of a... 

Chromatographic methods, notably hplc, are available for the simultaneous deterrnination of ascorbic acid as weU as dehydroascorbic acid. Some of these methods result in the separation of ascorbic acid from its isomers, eg, erythorbic acid and oxidation products such as diketogulonic acid. Detection has been by fluorescence, uv absorption, or electrochemical methods (83—85). Polarographic methods have been used because of their accuracy and their ease of operation. Ion exclusion (86) and ion suppression (87) chromatography methods have recently been reported. Other methods for ascorbic acid deterrnination include enzymatic, spectroscopic, paper, thin layer, and gas chromatographic methods. ExceUent reviews of these methods have been pubHshed (73,88,89). 

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