Thiaminase of Bacillus aneurinolyticus

Toxopyrimidine (pyramin lOlO) may be obtained from thiamine (vitamin Bi 1011) by acidic hydrolysis or by treatment with the thiaminase of Bacillus aneurinolyticus. Toxopyrimidine produces convulsions and death in rodents as do analogues, e.g. 2,4-dimethylpyrimidin-5-ylmethanol (1012) (65JMC750), but the effect is minimized or even... 

Enzymes destroying thiamine have been encountered in some species in a number of fresh-water fish, in very few salt-water fish, in some shellfish and Crustacea, in bracken ferns and in three species of intestinal bacteria. They have all received the name of "thiaminase , but it is very probable that we have to do with several, different, enzymes >23 They all bring about a fission of the thiamine molecule, liberating the thiazole part, but only in one case, that of thiaminase of Bacillus aneurinolyticus Kimura el Aoyana, reasonable proof has been obtained of a hydrolytic fission, yielding both moieties of the vitamin molecule z-methyl-q-amino-S-hydroxymethylpyr-imidine and 4-methyl-5(/ -hydroxy)-ethylthiazole. 

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See also in sourсe #XX -- [ ]

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