Thermodynamics cold denaturation temperature

Another interesting feature of this extended p-sheet model can be seen in Figure 3b, where we show the effect of temperature on the CD spectrum of Peptide 5. Curiously, when the sample temperature is decreased (to 5 C), the spectrum takes on more of a "random coil character (only 20% p-sheet) but, when the temperature is increased to 85 C, the spectrum exhibits significantly more P-sheet character (38% p-sheet). In other words, high temperatures induce structure and cold temperatures reduce structure. We believe that this represents an excellent example of cold denaturation (19), and it suggests that the thermodynamics of p-sheet formation may be more complex than currently appreciated. 

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