The Use of Other Commercially Available Proteases

Two other proteases have been commercialized by Sigma and used for such experiments. They both are extracts from fungi protease from. Rhizopus species (type XVIII), EC 3.4.23.6, optimum pH 3 and protease from Aspergillus saitoi (type XIII), EC 3.4.23.18, optimum pH 2.8. Several others were purified or overexpressed by groups developing the approach. 

E giKYL ME ESYVRE jS KQVSFGAKGNGgYAN MSIKKELEAA EVKGIDmS RSY GgF ASSFIGLA  

YGTM HSTG I gTVPGQN VA CSGT IADEKNGGYLV GLTDYIFSWSMSPAEPD ILYWVggPEH Ig E  

Peptides with pepsin digestion Peptides with type XIII digestion Peptides with type XVIII digestion 

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